Cajanus Cajan Lab Report

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2.3. Extraction of the flavonoids fraction of Cajanus cajan (FFCC) The Cajanus cajan L. seeds were air dried at 10% moisture, then powdered and preserved in a refrigerator at -80°C. Five hundred gm from the powdered seeds were defatted with hexane, then extracted with methanol (MeOH - 70%) four times, then concentrated under vacuum to yield 37 g. Polyamide and sephadex LH-20 chromatography columns were used to fraction the MeOH (70%) extract of Cajanus cajan. Seven flavonoid fractions were collected and identified as follows: apigenin, isorhamnetin, luteolin, orientin, quercetin, quercetin-3-O-B-D-glucopyranoside, vitexin. The pharmacological composition of the extracted flavonoids and their antioxidant activities were described in our coauthors published data [24]. 2.4. Animals and treatments…show more content…
Mice were housed in groups (5 animals/ group) under the standard conditions of the NRC animal care Ethical Committees by reserving the standard temperature (22-24°C), humidity (60±5%) and 12h day/night cycle. Food (purchased from the Pyramid Poultry Co., Giza, Egypt) and water were given ad libitum. Mice were simultaneously fed with FFCC orally by gavage and/or CP intraperitoneally (i.p.) for 24h. The experimental design was done as follows: The 1st group was treated with vehicle as negative group. The 2nd group was treated with FFCC at 200 mg/kg b.wt. The 3rd group was treated with CP at 20 mg/kg b.wt. The 4th, 5th and 6th groups were simultaneously treated with FFCC, at variable concentrations 50,100 and 200 mg/kg b.wt respectively, followed by CP. Samples were collected 24h after the last

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