Cajanus Cajan Lab Report

716 Words3 Pages
2.3. Extraction of the flavonoids fraction of Cajanus cajan (FFCC) The Cajanus cajan L. seeds were air dried at 10% moisture, then powdered and preserved in a refrigerator at -80°C. Five hundred gm from the powdered seeds were defatted with hexane, then extracted with methanol (MeOH - 70%) four times, then concentrated under vacuum to yield 37 g. Polyamide and sephadex LH-20 chromatography columns were used to fraction the MeOH (70%) extract of Cajanus cajan. Seven flavonoid fractions were collected and identified as follows: apigenin, isorhamnetin, luteolin, orientin, quercetin, quercetin-3-O-B-D-glucopyranoside, vitexin. The pharmacological composition of the extracted flavonoids and their antioxidant activities were described in our coauthors published data [24]. 2.4. Animals and treatments…show more content…
Mice were housed in groups (5 animals/ group) under the standard conditions of the NRC animal care Ethical Committees by reserving the standard temperature (22-24°C), humidity (60±5%) and 12h day/night cycle. Food (purchased from the Pyramid Poultry Co., Giza, Egypt) and water were given ad libitum. Mice were simultaneously fed with FFCC orally by gavage and/or CP intraperitoneally (i.p.) for 24h. The experimental design was done as follows: The 1st group was treated with vehicle as negative group. The 2nd group was treated with FFCC at 200 mg/kg b.wt. The 3rd group was treated with CP at 20 mg/kg b.wt. The 4th, 5th and 6th groups were simultaneously treated with FFCC, at variable concentrations 50,100 and 200 mg/kg b.wt respectively, followed by CP. Samples were collected 24h after the last
Show More
Related

  • Essay On Herbal Medicine

    5238 Words  | 21 Pages

    INTRODUCTION 1.1 Herbalism Herbalism is one of the traditional or folk medicine practice which is also known as medical herbalism, botanical medicine, herbology, herbal medicine, and phytotherapy. The scope of herbal medicine is extends from plants to fungal and bee products, as well as animal parts (Acharya and Shrivastava, 2008). Study about traditional use of medicines is recognized as a better way to learn about the potential future medicines. Around 80 of the modern medicines used were derived

    Read More

More about Cajanus Cajan Lab Report

Open Document