Singular Concentration Of Riboflavin

1510 Words7 Pages
Colorimetry Prepared by: Kevin Douglas Ellsworth Prepared for: Dr. Silviu Faitar Date: 10/02/2014 BIO 603L Lab Group: A/L3 Colorimetry I. INTRODUCTION/OBJECTIVE The purpose of this experiment is to recognize a singular concentration of riboflavin using colorimetry. The colorimetry measurement will provide an approximation of an unknown concentration of riboflavin. Enzymes that catalyze oxidation-reduction reactions using either or flavin adenine dinucleotide (FAD) or flavin mononucleotide (FMN) as coenzyme are flavoproteins. These forms are involved in several flavoprotein enzyme reactions. FAD and FMN are resultants from the vitamin riboflavin. Riboflavin or vitamin B2 has distinct properties of being soluble in water and predominantly yellow. One reaction of this holoenzyme involves hydrogen-transfer when united with its related apoenzyme and a significant example is the production of ATP occurring from electron transfer reaction. In order to…show more content…
The now diluted 50 mL solution of 5.23 X 10-5 M riboflavin solution could now be read on the colorimeter to get the best absorbency readings. The readings led to the values in Table 1. The absorbance of riboflavin at the concentration of 5.32x10-5 M illustrates the highest point where absorbance took place, and these points’ help determined the Lambda Max which is the wavelength of 445 nm between 440 nm and 450 nm. The values from Table 1 could now be plotted on Graph 1 which plots the Wavelength vs the Absorbance, to further illustrate how the 2 points can help to determine Lambda Max. Looking at the graph, it reciprocates the Lambda Max Graph 1 values by showing how the points make a plateau and rapidly decrease exemplifying that these points were the maximum values in which absorbance takes place before steadily

    More about Singular Concentration Of Riboflavin

      Open Document