Polymerase Chain Reaction Essay

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Abstract The experiment is to identify the guilty suspect that is present at the crime scene by comparing with the DNA samples. Polymerase Chain Reaction(PCR) is used to amplify the small amount of Deoxyribonucleic Acid (DNA) for forensic or genetic studies, which require necessary product and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using an electric current applied to the gel matrix, which causes the DNA samples to move towards the positive charge. The results obtain from the gel electrophoresis able to concluded the which suspect have been at the crime scene by analyzing against the…show more content…
In order to determine the DNA, the fragments are being placed in the agarose gel, which electrical charge is being sent through and pushing the negatively charged (black) molecules towards the positive side (red) when the gel is immersed in the buffer. By placing the molecules into the gel and applying an electric current, it would cause the molecules to move due to the different rates, that is usually determined by the mass or sizes. Objective The purpose of the experiment is to identify the guilty suspect who is present at the crime scene by obtaining and analyzing the DNA samples which were obtained from the crime scene and compare against the four main suspects. If results of the DNA obtain from the evidence found at the scene of the crime matches the DNA of the suspect, the person would be charged as a potentially guilty person. Methods and Materials The whole experiment is being divided into two phases. For the first phases, which is obtained the DNA sample of the suspect and for analyzing. Firstly, gloves are required to be worn to avoid contaminating the reaction mixtures. Five PCR tubes were being used and labelled as CS, A, B, C, D. The labeling of the tubes helps to prevent mixing up the different DNA samples together, where CS represent crime scene and A, B, C, D represent the

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