Paper Chromatography Lab Report

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Chromotography Myah Lanoux 2337 – General Chemistry II Laboratory October 10th, 2014 Introduction The purpose of the chromatography lab is to separate mixtures using horizontal paper chromatography and ascending chromatography. Using these methods the individual colors within a black dye will be separated and two unknown metal ions in can be identified from a solution. Skills required for this lab are analyzing, identifying unknown separable mixtures. Paper chromatography works majorly on capillary attractions. Capillary attraction depends on adhesive and cohesive forces. This report describes the experiment conducted using paper chromatography to identify an unknown mixture. Comparing four known metal ions with an unknown mixture in order to identify the unknown.. The experiment will also help master the technique and be able to analyze the movements made by both unknown mixture and the known…show more content…
35 ml of propanone and 10 mL of 6 molar HCL should be mixed and added to the 600 mL beaker. A large piece of chromatography paper should be used, place a pencil line 2 cm from the bottom and 1 cm from the top. Use capillary tubes to place one dot of each metal ion on the bottom line as well as a dot of unknown. Label each ion at the top of the sheet. Record any observations. Bend into cylinder and staple before placing in the 600 mL beaker. Cover with Para film and allow to develop. Mark the solvent front with a line and dry. Measure and record distances for ions present. Spray paper with concentrated aqueous ammonia, allow drying, record observations and measure ions present. Spray paper with 1.0% dimthylglyoxime, allow drying, record observations and measure ions present. Finally spray with 1.0% potassium hexacyancferate (II) allow drying, record observations and measure ions present. Use observations and measurement to calculate Rf and determine the identity of the unknown
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