Laccase Lab Report

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In 1894, laccase was first studied by Gabriel Bertrand in Chinese lacquer tree sap, where it serves in the formation of lacquer (hence the name "laccase"). Laccase enzymes belong to the group of diphenol oxidase family (E.C. 1.10.3.2.) acts on phenolic and non phenolic substrates due to wide substrate specificity. Laccases are widely distributed among plants, fungi, bacteria and insects. Rhus vernicifera, well studied plant source for laccase, cell culture of Acer pseudoplatanus, xylum tissue of Pinus taeda and Populus euramericana, green shoots of tea and leaves of Aesculus parviflora, cabbage, turnips, apples, beets, potatoes, pears, asparagus have been reported laccase activity. Laccase have role in plants for lignin synthesis, in wounding responses, in cell wall structure maintenance and integrity. Laccase involve in lignin synthesis pathway where it oxidizes precursors of lignin i.e. monolignols into free radicals and polymerize them into lignin. Wounded…show more content…
In short laccase catalysis occurs in three steps: (1) type I Cu reduction by substrate; (2) type I Cu transfers electron to the type II Cu and type III Cu trinuclear cluster; (3) at the trinuclear cluster reduction of oxygen to water molecule [9]. Actual mechanism is, in the presence of reducing substrate, T1 copper atom oxidizes first electron of the substrate and transfer it toward the trinuclear cluster. It is believed that the carbohydrate portion of the molecule ensures the conformational stability of the globule and protects it from proteolysis and inactivation by radicals [. Bolobova, A.V.2002, Yoshitake, A 1993, Ko, E.-M
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