3.1 Introduction This chapter discussed about the flow bench works of this analysis study of bacterial infection among pregnant and non-pregnant women. In the sub chapter 3.2 had explained about how to sample the specimen from the subject which was pregnant and non-pregnant women. Specimen that needs to be taken was urine. In sub chapter 3.3 shows the steps of dilution hence this process was repeated to make successive dilutions from 1ml of urine from the specimen taken before. Figure shows the flow chart of this project. From the figure, it shows that the initial step to start this project was to do literature review about earlier research or article related to the topic. This is as preparation before start…show more content… Prepared two types of agar for the project in the lab as when the real experiment later using urine specimen would give better outcome. The two agars are Eugonic agar and basic agar powder. The purpose is to compare between those agars when streaking the bacteria later. Basically, Eugonic agar has a purpose to yield bacteria in a eugenic growth. This pouring plate procedure has their own specific weight according to their respective types of agar. Generally, the steps are the same but the weights are…show more content… This procedure distinguishes between Gram positive and Gram negative groups by coloring these cells either red or violet. Hence, the purpose of having gram staining is that it ability to determine relevant treatment for an infection.
Need to place a glass slide with heat fixed smear on staining tray. Then, gently flood smear with crystal violet and let stand for 1 minute. Afterwards, tilt the slide slightly and gently rinse with tap water or distilled water using a wash bottle. Once again, gently flood the smear with iodine and let stand for 1 minute. Tilt the slide slightly and gently rinse with tap water or distilled water using a wash bottle. The smear will appear as a purple circle on the slide.
Decolorize using 95% ethyl alcohol whilst tilting the slide slightly and apply the alcohol drop by drop for 5 to 10 seconds until the alcohol runs almost clear. For precaution, be careful not to over-decolorize and immediately rinse with water. Gently flood with safranin to counter-stain and let stand for 45 seconds. With the same way, tilt the slide slightly and gently rinse with tap water or distilled water using a wash bottle. Then, blots dry the slide with bibulous paper and view the smear using an inverted microscope under