Clarias Batrachus Case Study

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SIGNIFICANCE EFFECTS OF OZONE AGAINST THE HEAVY METAL AND UV-A ON HEMETOLOGICAL AND BIOCHEMICAL FACTORS OF Clarias batrachus (WALKING CAT FISH) RaghuRam Ganesh1, Gnana Veera Subhashini*2, Karthik Kumar2, K.Renugadevi1. 1. Department of Biotechnology, Sathyabama University Jeppiaar nagar, Sholinganallur Chennai 600119, Tamilnadu, India. 2. Department of industrial and applied Biotechnology Baba Clinical And Genomic Research Centre, Phase-II, Ticel Biopark, Taramani, Chennai 600113, Tamilnadu, India. Corresponding author: Dr. Gnana Veera Subhashini Email id:dr.ngvsubhashini@bcgrc.com ABSTRACT: In the present work, the effects of lead under the exposure of various environmental factors on walking catfish, Clarias batrachus was divulge in terms…show more content…
UV stress will result in inedible replacement species or a significant decline in productivity [12]. Blood parameters truly reflects physical and chemical changes occurring in organism [10]. Walking catfish, Clarias batrachus are mostly encountered in muddy or swampy water of high turbidity [13]. They have high intolerance level and also they can adapt several physiological changes such as gills becoming highly vascularized (tree-like) organs acting as accessory breathing structures [7]. Thus walking cat fish is chosen for this study by exposing them to different environmental…show more content…
Experimental aquaria was fitted above 15cm from the water level [6]. 2.5 HEMATOLOGICAL AND BIOCHEMICAL ANALYSIS: After the vertical positioning of the fish, the lack of movement was noticed, the fishes were removed immediately and the blood was drawn from the caudal vein into EDTA and normal vacutainer. The hematological and biochemical analysis such as total cholesterol, triglycerides, High-density lipoprotein (HDL), Low-density lipoprotein (LDL), Very low-density lipoprotein (VLDL), total cholesterol/HDL, Total RBC’s, Haemoglobin (Hb), Packed cell volume (PCV) and Blood Glucose were analysed in auto analyser 2.6 STATISTICAL ANALYSIS: The hematological profiles were analysed statistically by calculating the Mean± Standard error. The data were analysed by the one-way ANOVA for significance [5]. Table 1.Hematological and biochemical analysis results (mean± standard error) of Clarias batrachus from Tamil Nadu- India, exposed to various environmental factors. Parameters Control Heavy metal Ozone+ heavy metal UV + heavy metal UV-Ozone + Heavy metal Total cholesterol (mgs/dl) 170.06±0.4797 *
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