Catechol Oxidase Lab Report
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When catechol (the substrate) and oxygen are combined, water and benzoquinone (a pigment that darkens fruits and vegetables) are formed. Thus, if the catechol oxidase, which acts as an enzyme to catalyze the above reaction, is successful, a darkening color change will occur. In Part C of the lab, test tube 2 turned a dark orange color, indicating a positive reaction. According to the lab handout, is it known that PTU combines with the copper (which acts as a cofactor, a substance that can activate an enzyme) in catechol oxidase to inhibits the enzyme’s activity. In Part D of the lab, when a test tube contained PTU, a reaction did not take place, because PTU combined with the copper in the enzyme and inhibited its activity. Therefore, a visible