Bacillus Endospore Lab Report

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Ultra Violet light has the power to kill microorganisms and cause mutations by acting on their DNA. In this lab exercise, we compared the effects that UV radiation has on Bacillus endospores and non-spore forming cells. Also, we conducted an experiment to see the effects of light and dark irradiation an organisms, using Serratia marcescens, after being introduced to the UV light. This was done in four different ways. In the 1st plate of the Bacillus endospore, we spread the organism over the agar plate, then exposed it to UV light for three seconds, without a lid on, and incubated it in the light. As a result, about 20 colonies formed. They were a medium sized and white. In the second plate of the Bacillus endospore, we used the same guidelines as the first, however, this was kept in the dark. No colonies formed on this plate and…show more content…
This also explains why no colonies were produced in the second plate that was irradiated in the dark. In the first plate of the Serratia marcescens organism, we exposed it to 254 um for 30 seconds, without a lid, but covered by a mask, and then was incubated in the light. About 75 to 100 colonies grew in the area that was irradiated. The outside part that was not exposed to the UV light was completely red. These results prove that the organism is somewhat resistant to UV light and went through light repair. The second plate followed the same guidelines as the first, yet was incubated in the dark. About 10 colonies had formed in the irradiated area, surrounded by a red area as well. By incubating this plate in the dark and there were colonies that still formed, we can determine that the organism is resistant to UV
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