Introduction After collecting a random air sample on an agar plate, the objective was to identify an unknown bacterium through a series of carefully executed metabolic tests. Not only was this to provide students with how to characterize an unknown sample but also it was a learning experience with every different test. Each experiment provided a new result gaining a step closer to identifying what the unknown was. The goal at the end was to have enough results and data to accurately determine what the sample
Title: Analysis of Mini unknown Summary: A series of test was done with Microbe 3B. A Gram stain was done and the sample was gram positive. A streak plate was completed revealing that the microbe was circular, convex, entire, smooth, yellow, and opaque. The last test completed was the MSA. The microbe was oxidase positive. Thus, Microbe 3B was Micrococcus luteus (Sarcina). (MMB,2015) Procedure 1 Gram stain: Purpose/ Introduction: The Gram stain reveals whether a microbe is gram positive
Isolation and Identification of Unknown Bacteria Microbiology is the study of organisms that are invisible to the unaided eye. Bacteria are a diverse group of microbes that have the ability to inhabit many different habitats, including soil, food, water, and human bodies (Collard, 1976). Among their beneficial uses is their use in the production of food, alcohol, and antibiotics. Not all bacteria are helpful to humans, however. Medical microbiology is an active field of study which deals with identifying
Mixed Unknown Bacteria Lab Report Biology 2460-002 Jamuna Kadariya November 24, 2014 Unknown number: thirty-four I. Abstract There are many types of bacteria and they produce different enzymes. However, some bacteria can produce same enzymes. In order to identify the particular enzymes produce by each bacteria specific test needs to be done. Among various types of enzyme producing bacteria, gram- negative and gram-positive bacteria are given priority in our experiment. There are four main types
of the chromatography lab is to separate mixtures using horizontal paper chromatography and ascending chromatography. Using these methods the individual colors within a black dye will be separated and two unknown metal ions in can be identified from a solution. Skills required for this lab are analyzing, identifying unknown separable mixtures. Paper chromatography works majorly on capillary attractions. Capillary attraction depends on adhesive and cohesive forces. This report describes the experiment
Acid-Base Titration Lab Report By Andjela Mandic 11/24/14 Period 1 Purpose: The purpose of this experiment was to find the molarity of a sodium hydroxide (NaOH) solution by titrating it with a Hydrochloric Acid (HCl) solution. Materials: Burets (50mL), Buret Stand, Buret Clamp, Graduated Cylinder (10mL), Erlenmeyer Flask (125mL), Index Card, Beakers (250mL), Safety Goggles, Aprons, 0.100M HCl (standard solution), NaOH (concentration unknown), Distilled Water, Indicator (bromothymol
Lauren Calso September 29, 2014 BIOL 240 Lab Report Gram Stain Introduction Various methods are used to identify unknown bacteria. A popular method to determine unknown bacteria is called a Gram Stain. A stain is coloring a specimen with dye. A Danish physician named Hans Christian Gram developed the Gram stain method in 1844. He studied at the University of Copenhagen and became a professor of Medicine. When differentiating between two types of pneumonia, he found that most bacteria are either
Looking Into Lactase Purpose Why am I doing this lab? What am I trying to find out? The purpose of this lab was to see in which environments (temperature and pH levels), the Lactex® tablet would be effective (if at all). In the first section the purpose of the lab was to discover what type of milks the three milks were. The experiment is done on cow milk, rice milk, and soy milk. The importance of the next section was to see the effectiveness of the tablet in different pH levels. The second section
Sarah Min CHEM 143A: Section A03 A10627548 April 14, 2015 Lab Report 1 - Recrystallization and Identification of Unknown Introduction The main concept in this experiment is recrystallization. Recrystallization is the process where the solute (a crystalline material) is dissolved in a hot solvent. The heated solution is filtered (generally via vacuum or gravity filtration) and is returned back to its solid crystalline state when the solution cools down. Recrystallization is best for isolating and
Objective: This lab was done to help understand restriction enzymes and how they work. As well as comprehend the correlation between these enzymes and DNA Fragments. This lab was conducted to estimate the size of unknown DNA fragment thru agarose gel and electrophoresis. Background: A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence. Restriction enzymes