Solid State Frermentation Process

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3.0 MATERIALS AND METHODS 3.1 SAMPLE COLLECTION Four different types of fruits selected in this study are Citrus sinensis, Psidium guajava L, Malus domestica and Ananas comosus. All fruits will be purchased at supermarket and bring to MSU laboratory for perform the solid-state fermentation method. 3.2 PROCEDURE FOR SAMPLE PREPARATION AND WASHING Four types of fruit will be transported to laboratory in plastic bags. According to the previous study, the fruit sample will be cut into smaller size and washed using distilled water. Then, swipe by using tissue which contains 70% alcohol to make sure the fruits is clean and dry from water. The weight of each fruit is approximately 100 gram per fruit. The fruits will then put inside the sterile 250…show more content…
In this study, the sample is the complex yield which produced after fermentation process. The yield sample will be prepared for perform all test regarding the parameters. For moisture content, the yield sample will be determined by using Standard Official Methods of Analysis of the AOAC (1990). This process will be involving the drying method and constant weight at 100 °C so the moisture content will be calculating as the loss in weight of the yield samples. The percentage of moisture content will be calculated using the standard formula. Then for the ash content, the total of ash will be determined by using Furnace Incineration described by AOAC (1990) which is use about 1.0 g of yield sample. This analytical method are created on the water vaporization and volatiles with burning or boiling organic substances in the presence of oxygen in the air to the carbon oxide at a temperature of 600 °C before calculate the ash percentage using standard formula. While for the fiber, it will be determined by using the method of AOAC, 1990 and the percentage of the crude fiber will be calculated as per the standard formula. For the total fat, it will be determined by using Soxhlet extraction for 4 hour starting with methanol and ethanol separately (Eromosele and Eromsele, 1994). The protein content of the yield samples will be determine by using the Microkjeldahl method of AOAC (1984), which involve the protein digestion and distillation. The percentage of nitrogen will be calculate to identify the amount of protein which presence inside the yield sample. Then, the total percentage of the carbohydrate content will be determined by using the difference method which involves the additional of the total values from protein, lipid, fiber, moisture and ash constituents of the yield sample. The value will be obtained in the percentage of carbohydrate

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