Protein Electrochemistry: Macromolecules
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ntroduction Proteins are macromolecules that makes up cells and are encoded by amino acids sequences.Measuring protein concentrations in an stunning diverse array experimental contexts,Spectrophotometer allow us to quantity protein because the color will change as the protein level changes.The Biuret reaction involves a reagent containing copper (cupric) ions in alkaline solution. Molecules containing 2 or more peptide bonds associate with the cupric ions to form a coordination complex that demonstrates a purple color to the solution with Amax = 540 nm. The purple color of the complex can be measured independently of the blue color of the reagent itself with a spectrophotometer Protein assays based on the Biuret reaction were developed