genetic information from one organism to another. In the Transformation Lab completed in class, bacteria was used to replicate the transformation process in combination with pGLO plasmid and Transformation Solution. pGLO is a plasmid that contains ori bla, ara, and GCF. To understand the process of transformation using a pGLO plasmid, one must be acquainted with the functions of the aforementioned properties. Within the pGLO, an ori is where DNA replication begins. bla is an enzyme that codes for
transformation is a form of genetic transformation and also the easiest to create within a lab due to the single cell nature of the bacteria used. For this particular experiment pGLO plasmid will be incorporated into E. coli bacteria which will give it genes that code GFP and beta-lactamase proteins into the modified genome of the bacteria. For the experiment two plates were mixed with and without the pGLO plasmid; one was the pGLO, LB nutrient broth mixed the ampicillin, and the other was the LB nutrient broth
these jellyfish to glow in the absence of light. pGLO has specific regulations for genes, which are able to control fluorescent protein expression in transformed cells. This is done by using the sugar arabinose. When the araC protein binds to the DNA sequence and is turned on in the presence of arabinose and activates it and it helps the araC bind to the RNA polymerase and transcribes the araA, araB, and araD enzymes. The coding sequence of the pGLO has been modified to include many of the inner workings
transformation procedure enables others to inserts various genes by recombinant techniques and to place the plasmid into a bacteria for a gene expression. In the lab experiment, the transformation of Escherichia coli with new DNA was explored to determine its different genetic information. In order to grow the bacterium the plate must contain a pGLO plasmid and must be expressing the ampicillin resistance protein β-lactamase. If arabinose is present, transcription of the gfp gene is enabled that results
The purpose of this experiment is to explore the understanding of bacterial transformation of plasmid DNA. Bacterial transformation is the technique of genetic transformation. In this lab the pGLO plasmid is pledged into E.Coli bacteria and adds genes encoded for GFP and beta-lactamase to transformed bacteria. To get the results of the experiment, the bacteria processed with plasmid were grown on two plates , one of which containing LB nutrient broth and ampicillin and another plate containing LB
The Transformation of Escherichia coli With pGalTM Sayan Martin General Biology II – M4L Dr. Anupam Pradhan September 20, 2015 Lab Partners: Sam Vertus Alyssa Ramlogan Priya Hammonds OBJECTIVE: To develop an understanding of bacterial transformation by plasmid DNA, and determine the transformation efficiency of a bacterial sample. INTRODUCTION: Transformation is an important biological mechanism for generating genetic diversity
Since 1970s, image retrieval is an effective research area. Image retrieval is a technique used for browsing, retrieving, and searching images from a huge database. Due to the advance technologies in the internet and digital imaging devices has been explosive growth of digital images. So, image retrieval technique is an efficient and effective for maintaining large database. There are two image retrieval schemes: text-based image retrieval and CBIR. Text-based image retrieval can be outlined back
Bacterial Transformation Alyssa Krajnik Lab TA: Qi Deng Lab Time: Thursday 4:30 Report Submitted: 09 APR 2015 Abstract: The purpose of this experiment was to observe the process of transferring genes from one organism to another using the aid of a plasmid. However, the results obtained from the experiment illustrated that the data collected was not reliable. The results indicated that not enough bacteria were transferred to the agar plates. Because of the errors that occurred in the experiment