Acetyl cysteine is used as a mucolytic agent to reduce the viscosity of secretions probably by means of splitting the disulphide bonds in mucoproteins. Acetyl cysteine in addition is able to promote the detoxification of an intermediate paracetamol metabolite and have a chief role in the supervision of paracetamol overdosage. It is in use for its mucolytic action in respiratory disorders coupled with productive cough.
Literature study reveals that numerous methods were reported for the determination of N-Acetyl cysteine.The evaluation includes colorimetric (Raggi et al., 1982),spectrophotometric (murty et al., 1977), Chemiluminescence (vinas et al.,1993), HPLC with electrochemical (William et al., 2007; Drozdz et al.,…show more content… 2.3. Standard solution preparation
Solution of standard were prepared by dissolving individually 10 mg of N-Acetyl cysteine and Taurine each in 10 mL of methanol to obtain a concentration of 1000 µg mL-1. The Standard stock solutions were suitably diluted with methanol to obtain the working standard solutions of both N-Acetyl cysteine and Taurine.
2.4. Preparation of sample solutions
Twenty tablets (Nefrosave) were weighed and grounded to fine powder. An accurately weighed powder sample equivalent to 100 mg of N-Acetyl cysteine was weighed, transferred to a 100 mL volumetric flask and the volume was made up to about 70 mL with methanol.The solution was sonicated for about 30 minutes, afterwards diluted to volume with the similar solvent and it was filtered through Whatman filter paper No.42.Working sample solutions were newly prepared by diluting appropriate volumes of the stock sample solution with methanol.
2.5. Optimised Chromatographic Condition
Appropriate volumes of standard and sample solutions (µL) were applied on to the HPTLC plates.The mobile phase comprising of n-butanol:acetic acid:water (8:0.5:1.5 v/v/v) was used in every chromatographic run. Ascending development method was carried out in twin trough chambers.The chamber saturation time for the mobile phase was 20 min at room temperature (25 ± 2ºC