Enamel Demineralization Case Study

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EVALUATION OF ANTIMICROBIAL PROPERTIES AND SHEAR BOND STRENGTH OF TRANSBOND XT ORTHODONTIC PRIMER WITH AND WITHOUT CHLORHEXIDINE INCORPORATION: AN IN-VITRO STUDY INTRODUCTION During orthodontic treatment with fixed appliances enamel demineralization commonly occurs at the interface between the bracket and the tooth surface. This is primarily caused by adhesion of cariogenic bacteria, such as Streptococcus mutans. Many efforts have been made to develop an antibacterial orthodontic adhesive system to prevent enamel demineralization at the interface. One approach is to incorporate antimicrobial agents like fluoride or chlorhexidine, into orthodontic adhesives by physical blending.1, 2 However, these agents have some disadvantages,…show more content…
Prevalence of white spot lesions among orthodontic patients ranges from 2-96 %. White spot lesions (WSL) are aggravated by presence of Streptococcus mutans due to plaque appearance on the enamel surrounding the orthodontic attachments.5 Hence to reduce the risk of demineralization and white spot lesions this study is being conducted by incorporation of CHX in Transbond XT orthodontic primer to study its effect on shear bond strength and bond failure patterns. AIM AND OBJECTIVES AIM To compare the anti-microbial properties and shear bond strength of Transbond XT orthodontic primer with and without chlorhexidine incorporation. OBJECTIVES 1. To test the anti-microbial properties of Transbond XT orthodontic primer incorporated with chlorhexidine by analyzing Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. 2. To compare the effect of Transbond XT orthodontic primer incorporated with and without chlorhexidine on shear bond strength and bond failure patterns. 3. To evaluate the effect of thermocycling on shear bond strength and extent of biofilm inhibition of Streptococcus Mutans. 4. To evaluate the extent of biofilm inhibition of Streptococcus Mutans by Transbond XT orthodontic primer incorporated with chlorhexidine. MATERIALS AND METHODS Inclusion Criteria: - Freshly extracted human first premolar teeth without cracks, caries, fracture,…show more content…
mutans will be carried out under strict supervision in the laboratory. Plates will be thermocycled in water to analyze the effects of water aging on antimicrobial activities and to simulate normal aging in the oral cavity. The thermocycling equipment ( Applied BiosystemsTM, Germany) will be set at 20-second dips to 5 seconds of transfer time. Five thousand cycles will be performed. After thermocycling, growth assays will be performed again under the same environment. Unstimulated whole saliva will be collected by the expectorating from healthy volunteers.7 Saliva samples will be centrifuged at 3500 X g for 10 minutes to remove any cellular debris and the resulting supernatant will be used after filter-sterilization for assessing biofilm formation of S. mutans. To assess biofilm formation, S. mutans UA159 will be grown in a semi-defined biofilm medium with 18 mM glucose and 2 mM sucrose as carbohydrate sources. For saliva coating, each primer-conditioned well will be conditioned with 60 µLof saliva. Biofilm formation will be quantified by measuring the absorbance of the solution at 600 nm using a spectrophotometer (ShimanduTM,

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