15 Daphnia magna were used in total for this experiment. Five Daphnia for each treatment to be tested were transferred by a wide pipette onto a petri dish and put on the Daphnia cooling chamber; the cooling chamber brought down the heart rate of the Daphnia. There was enough water added with the Daphnia in order to ensure survival. To allow slight movement, yet restrict the Daphnia from swimming cotton wool put on the animal. To maintain a temperature conducive to the heart rate measurements, the