Catalase Speed Up Reaction Rates

1298 Words6 Pages
Abstract: Catalase speed up reaction rates in substance or even molecules. Factors such as temperature and substrate concentration can also contribute the speeding up of reaction rates. Previous research has shown how these factors influence the reaction time of particular substances. This is most likely because catalase speeds up reactions along with the contributing factors, making the reaction happen much quicker. In our experiment, we tested five different substrates (0.8%, 0.4%, 0.2%, 0.1%, and 0.0%) with a phosphate buffer and we also tested the phosphate buffer at different temperatures in degrees Celsius (boiled-100, warm water bath-40, room temp-25, iced-0) mixed with a 0.8% substrate to try and see the effects of these outside factors…show more content…
These specific enzymes are known as catalysts. Enzymes also break down molecules which are called substrates. Many believe that because catalase has such a fast reaction rate, they have a separate entrances and exits to keep the incoming hydrogen peroxide and the leaving oxygen. (Loewen, Klotz, and Hassett 2000) There are many factors that can affect the reaction rate such as temperature, concentration levels, pH levels and etc. In this experiment we tested five different substrates that had different concentrations and then mixed with them catalase buffer solution to see if the reaction time would change or be affected. Buffers are solutions that are supposed to keep a reaction stable and keep make them resist change. In experiment one, we expected that by increasing the concentration of the substrate, the reaction rate would also increase. In experiment two we test the effects of temperature change of the catalase to the reaction time. In the sensitive modification of the Catalase Assay with the Clark Oxygen Electrode experiment done by Rio, Ortega, Lopez and Gorge, they study a similar affect by trying to see how much oxygen was released with an oxygen-free buffer (Gorge 1977); this is very similar to what we did with our control variable and not adding a substrate concentration. We expected that increase in temperature would increase the reaction rate as well. In a similar experiment done by George P. Kubica and Gleason…show more content…
The results shown in the result section supports both of the hypothesizes. Our results are supported by the experiment done by Loewen, Klotz and Hassett showing that the higher the concentration of the substrate the faster the reaction meaning oxygen is leaving faster and it is able to leave faster because the catalase enzyme has an “entrance” and an “exit” so the leaving oxygen does not interfere with the entering hydrogen peroxide. (Loewen, Klotz, Hassett 2000) The same situation occurs in experiment two when the temperature affects were

More about Catalase Speed Up Reaction Rates

Open Document