Bacteriophages Lab Report

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Abstract: This experiment will be about the log graph of mycobacteria. We will be testing out to see whether mycobacteria will have a logarithmic graph. Meaning, the graph will have a stationary phase, an exponential phase, a stationary phase, and finally a death phase. The first phase would be were the bacteria are getting use to their environment. Followed by a exponential phase where the cells start to divide and there a huge burst of cells, this phase is also called the burst phase. Then comes the stationary phase where the nutrients in the medium have decreased and there is no upward growth of cells. Then finally comes the death phase where we have a decline of mycobacteria. This is where there is not enough nutrients and they organism…show more content…
Bacteriophage T4 selectively find and infects bacterial species such as E. coli. The bacteriophages has a very complex structure that includes a polyhedral head, sheathed tail, base plate, and also attached tail fiber. The T4 bacteriophages uses its tail fibers for absorption. Then it attaches its base plate protein as well as injecting the host cell with its DNA. Once the bacteriophage has enough virus proteins and virus DNA synthesized, new virus start to be assembled. The new viruses then are released and can start to infect other bacterial cells. The life cycle of the bacteriophage can be seen by following a one-step growth curve. What happens is a broth culture of a bacteria that can be infected is mixed with the proper quantity of bacteriophages. Once this is set up then we start with the initial latent period. The latent period is where no viruses are released. This is the period where the bacteriophages has taken over the metabolic systems of the host cell, where it start to synthesize new viral DNA. Once this period is complete then we move onto the eclipse period, which a phase between the uncoating and assembly, where there are no bacteriophages. Then from this hibernation a sudden bust of a large amount of bacteriophages are released. This is called the bust size, and it can vary with the type of virus that we are dealing with. Bacteriophages are very small and hard to

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