Mannitol Salt Agar Essay

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1. What are the differences between selective and differential media? Differential media types are those who distinguish microorganisms from each other based on growth characteristics evident once growth on specific media types. Whereas selective media types are developed to support, the expansion of one group of organisms but inhibits another. 2. What is MSA used for? Mannitol Salt Agar (MSA) commonly used for differential and selective media 3. How does MSA work? MSA encourages the expansion of a group of certain microorganism live in areas of higher salt concentration like staphylococcus species. 4. What color would E. coli grown on MSA be? Escherichia coli is a gram negative bacterium that don't grow on MSA plates, then it's not halophilic bacterium, that has no color, 5.…show more content…
What color would staphylococcus epidermidis grown on MSA be? Staphylococcus epidermidis is gram-positive bacterium and is in a position to grow on the MSA agar then its halophilic bacterium with no yellow halo that indicates that it's mannitol non-fermenting colony (pink). 6. What is EMB used for? Eosin methylene blue agar (EMB) is used for differential and selective media. 7. What does the eosin dye in EMB do? Eosin dye found in EMB agar, which allows the differentiation between lactose fermented and non-fermented. Eosin additionally acts as a pH indicator that measures the amount of acid production to the fermentation of lactose. Eosin is a stain that used for staining the cell to look at the structure and therefore the range of cells. 8. What color would E.coli grown on EMB appear? Escherichia coli is a gram negative bacterium and is in a position for growth of colonies on an EMB agar with a green-metallic sheen of colonies, 9. What would staph grown on EMB look

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