Ethyl Laurate Lab Report

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Ethyl and hexyl esters are among the important and versatile components of natural flavours and fragrances. Ethyl Laurate is widely used in food , aromatics, flavours, pharmaceuticals, detergents and cosmetic industries[1]. Traditionally, most of the flavour compounds are produced by chemical synthesis or extraction from natural sources. Recently most esterification reactions are catalyzed by inorganic acid, solid acids, Lewis acid, ion-exchange resin and metallic compounds [2,3]. These methods required very high temperature and produces hazardous by-products. In contrast with these chemical synthesis, biocatalysts allow synthesis of esters to be performed at moderate temperatures, atmospheric pressure. For application in the food flavour industry…show more content…
But reaction required high temperature and pressure. In comparison enzymatic reaction are carried out at mild temperature and pressure. There were many literature on lipase catalysed synthesis of fatty acid ethyl ester. M.L.M. Fernandes et al reported a 92% yield of ethyl laurate using Lipolase, a commercial preparation containing the lipase from Thermomyces lanuginose[20]. Solarte et al reported biocatalytic synthesis of ethyl laurate with a 95% yield after 24 h of reaction by using the whole cell lipase from A. flavus [21]. Kanwar et al reported 66% conversion of ethyl laurate in n-nonane at 65˚C after 15 hours by hydrogel immobilized lipase of Bacillus coagulans MTCC-6375…show more content…
fully baffled mechanically agitated reactor of 50 ml capacity, equipped with 3 baffles and a 3-bladed turbine impeller. The whole reactor assembly was immersed in a thermostatic water bath. The desired temperature was maintained with an accuracy of ±1˚C. A typical reaction mixture consisted of Ethanol (50 mM) and lauric acid (50mM) without any solvent mixture. The reaction mixture was agitated at 60˚C for 10 min at a speed of 200 rpm. To start the reaction fermase CALBTM10000 (2 % w/w of substrate) of immobilised enzyme and molecular sieves (12 % w/w of substrate) were added in the reaction mixture. Liquid samples free catalyst were withdrawn periodically and analysed by titrimetric analysis. The product were confirmed by GC and GC-MS. All experimental data are average values within an error of

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